Configurations with up to 13 fluorescence parameters and 3 physical light scattering parameters
Choice of lasers with 365, 375, 405, 488, 532, 561, 594, 638, 640 nm
Maximum acquisition rate up to 25,000 signals/s
Scatter particle size: 0.1 – 100 µm
Fluorescence sensitivity: < 100 MESF (FITC), < 50 MESF (PE)

CyFlow Space – its flexibility gives you the space you need for your work

The CyFlow Space is a high-performance multi-laser flow cytometer, which offers the most flexible, simple and reliable features for routine and research work.

It is a compact multi-colour instrument for the analysis of individual cells and microscopic particles in suspension. The advanced flow cytometry technology of the CyFlow Space covers a wide range of different applications.

Fluorescence excitation by one to five light sources covering a range from ultra-violet to red excitation lines. o Analysis of up to 13 fluorescence parameters and three physical light scattering parameters from near UV to infra-red light.
Analysis of cell and particle concentration by True Volumetric Absolute Counting (TVAC).
Cell sorting by an optional cell and particle sorter.
Fixed standard configurations and flexible customised configurations tailored to the user´s requirement.
Walk-away automation by sample auto-loading from 96- and 384-well plates.

More details

FloMax flow cytometry data acquisition and analysis software

The Windows™-based FloMax software includes multiple online gating, logical gates, and colour gating. N-colour online and offline software compensation is another standard feature of the FloMax software. Data may be acquired with compensation or as uncompensated raw data. Either way, compensation can be done, undone, or changed after analysis on stored data. User-selectable statistics are calculated on subset populations identified by regions that are created by the user with easy-to-use drawing tools, and combined using simple, logical formulas. Other sophisticated features include cell cycle analysis and peak analysis. Custom reports are quickly and easily generated from the acquired data using the integrated, macro-driven interface with Microsoft™ Word and Excel. FloMax data files are stored in FCS standard format and are therefore fully compatible with other commercial flow cytometry software.

The FloMax software is pre-installed on the Windows™ PC that is delivered as part of the CyFlow Space systems. Three additional licences for offline copies of the FloMax software are also included free of charge, which offers a cost-effective solution for working groups with the need for offline data analysis software.

Technical specifications

Light sources	Up to five light sources Blue solid state laser: 50mW fix or 200mW adjustable at 488nm Red diode laser: 25mW at 638nm or 40mW at 640nm Violet diode laser: 100mW at 405nm Green DPSS laser: 30 or 100mW at 532nm Yellow laser: 100mW at 561nm High-power UV LED: 365nm Ultraviolet diode laser: 60mW at 375nm Orange diode laser: 50mW at 594nm
Optics	Modular optical system with up to 16/eight optical parameters with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL13 Exchangeable optical filters Colour CCD camera for sample flow monitoring
Flow system	Quartz flow cuvette for laminar sample transport and hydrodynamic focussing True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement Computer-controlled precision syringe pump, speed continuously adjustable from 0-20 µL/s
Software	Microsoft Windows™ operating system with full network support, data analysis and real-time data display Storage of instrument settings, screen layout templates, panels, gates and compensation matrices Flow Cytometry Standard (FCS) data format for storage of original and evaluated data Automated reporting of statistics, graphics and instrument settings to Word or Excel
Dimensions	W 560mm x H 300mm x D 650mm