Sysmex Europe
Menu

Please select your country. This will keep you up to date with your country-specific information.

CyFlow Cube 8

  • Configurations with up 8 optical parameters (up to 6 colours)
  • Choice of lasers with 375, 407, 488, 532, 561, 594, 638, 640, 785 nm
  • Particle size: 0.1 – 100 µm
  • Fluorescence sensitivity: ≤ 100 MESF (FITC) | ≤ 50 MESF (PE)
  • Optional CyFlow Robby 8 Autoloading Station

Documents

All
  • All
  • Brochure
  • Literature
  • Images
Documents

High performance flow cytometry

High-performance flow cytometry

The CyFlow Cube 8 is a compact flow cytometer for the analysis of single cells and microscopic particles with a high grade of integration. The CyFlow Cube 8 offers the unique combination of a truly stand-alone system of a small footprint with a modular configuration concept with up to 6 colours. The easy-to-use CyFlow software provides control of the instrument, data acquisition, analysis and storage. The possibility of customised instrument settings facilitates switching between different applications. CyFlow Cube 8 offers True Volumetric Absolute Counting (TVAC), which allows the display of particle concentrations for any subsets of cells without the need for reference beads, even if defined by a gate at a later time after the acquisition.

* Optional CyFlow Robby 8 Autoloading Station for well plates and tubes

* Optional CyFlow Sorter for closed, non-destructive cell and particle sorting with high purity

Flow Cytometry

Software

Software

CyFlow: Intuitive software for CyFlow Cube 8

The newly designed, intuitive CyFlow acquisition and analysis software offers the measurement of millions of cells at your fingertips.

  • Quickly choose your plots for analysis from all of the combinations shown in Preview Mode.
  • Easily compare plots with the Overlay Wizard, for single or dual parameters.
  • Share and customise your results within your research organisation with the Report Generator.
  • Change your view with Zoom and V-Log Scale; you are even able to gate in Zoom Mode.
  • Compensate rapidly with the Compensation Wizard.
  • CyFlow optimally supports the True Volumetric Absolute Counting of the CyFlow Cube 8, displaying particle concentrations for any subsets of cells, even if defined by a gate at a later time after the acquisition.
Specification

Specification

CyFlow Cube 8 

Light sources:

Up to four light sources (three lasers + high-power UV LED)

  • Blue laser: 50mW at 488nm, adjustable to 200mW at 488nm
  • Red laser: 25mW at 638nm, 40mW at 640nm
  • Violet laser: 100mW at 405nm
  • UV laser: 60mW at 375nm
  • Green laser: 30mW at 532nm, 100mW at 532nm
  • Yellow laser: 100mW at 561nm
  • Orange laser: 50mW at 594nm
  • High-power UV LED: 365nm

 

Optics:

  • Modular optical system with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL6
  • Exchangeable optical filters

 

Flow system:

  • Quartz flow cuvette for laminar sample transport and hydrodynamic focussing
  • True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
  • Computer-controlled precision syringe pump, speed continuously adjustable from 0-20 µL/s

 

Software:

  • Windows™-based FCM software CyFlow for real-time data acquisition, real-time data analysis and real-time data display
  • Easy set-up of experimental templates (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data

 

Dimensions:

  • Stand-alone instrument: W 500mm x H 370mm x D 470mm
  • With Autoloading Station: W 840mm

 

CyFlow Cube 8 Sorter 

General facts:

Configurations with up to five optical parameters (up to three colours)

Sterile sorting by closed fluidic system

Optional CyFlow Robby 8 Autoloading Station for well plates and tubes 

 

Light sources:

Up to two simultaneous light sources (lasers) 

  • Blue laser: 50mW at 488nm, adjustable to 200mW at 488nm
  • Red laser: 25mW at 638nm, 40mW at 640nm
  • Violet laser: 100mW at 405nm
  • UV laser: 60mW at 375nm
  • Green laser: 30mW at 532nm, 100mW at 532nm
  • Yellow laser: 100mW at 561nm
  • Orange laser: 50mW at 594nm

 

Optics:

  • Modular optical system with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL3
  • Exchangeable optical filters

 

Flow system:

  • Quartz flow cuvette for laminar sample transport and sorting process
  • Completely closed and integrated fluidic system
  • True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
  • Computer-controlled precision syringe pump, speed continuously adjustable from 0-20 µL/s

 

Software:

  • Windows™-based FCM software CyFlow for real-time data acquisition, real-time data analysis and real-time data display
  • Easy set-up of experimental templates (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data

 

Dimensions: 

 

  • W 500mm x H 370mm x D 470mm (without sorting module)

 

 

Upgrade

Upgrade

CyFlow Robby 8 Autoloading Station 

 

General facts:

  • Protective housing with no light incidence 
  • Flexible well/tube measurements

 

Microplates and tubes:

  • 48- or 96-well plates; V-, U- and flat-bottom are fitting (max.2) 
  • Plates meeting the standards ANSI/SLAS (1-2004 through 4-2004) 
  • 2mL-tubes (max. 120)

 

Sample volume:

  • Flexible choice of sample volume up to 180 µL for plates and 2,000 µL for tubes

 

Speed:

  • Continuously adjustable from 0.2 µL/s to 10 µL/s
  • Approx. 1h for a 96-well plate (speed 2 µL/s, sample volume 20 µL)

 

Software:

  • CyFlow Windows™-based software

 

Dimensions:

  • W 370mm x H 370mm x D 332mm
Application

Application

Absolute cell and particle counting
Cell and particle sorting

Microbiology: 

  • Yeast viability and fermentation process control 
  • Water quality analysis 
  • Absolute counting of bacteria and other microorganisms 
  • Viability of bacteria and other microorganisms 
  • Quality control in dairy industry and milk products 
  • Marine biology and algae 
  • Agrosciences and aquaculture 
  • Quality control in cosmetics

 

Industrial applications: 

  • Bioreactor process optimisation 
  • Quality assurance in food and beverage industry 
  • Biomonitoring 
  • Biofuels

 

Cell biology: 

  • Cell cycle and DNA analysis 
  • Viability and live/dead analysis 
  • Cell proliferation
  • Apoptosis 
  • Cancer research 
  • Toxicology
  • Cytokines and bead-based assays 
  • Cell signalling 
  • Plant biology 
  • Detection of ploidy level 
  • Plant genome size and DNA analysis 
  • Biomedical research 
  • Animal models 
  • Stem cells 
  • Immunology 
  • Hematological disorders 
  • Platelets
Complementary products

Complementary products

Sample preparation

Contact

Sysmex Europe GmbH

+49 (40) 527 26 0
+49 (40) 527 26 100
info@sysmex-europe.com
Bornbarch 1
22848 Norderstedt
Germany

Create Pdf
Enjoy our Media Centre
All
  • All
  • Documents
  • Podcast
  • Images
  • Videos
See our collection
Find your job