FIT screening

Colorectal cancer (CRC) is one of the most frequently diagnosed cancers. The good news is that CRC incidence and mortality can be reduced significantly if detected early enough.

Faecal immunochemical tests (FIT) are non-invasive and can detect blood in stool invisible to the naked eye. Due to its simplicity, FIT is currently considered the best non-invasive test for CRC screening.

Invest a little time in your own health by taking the FIT to prevent or detect colon cancer early on.
For further information, please visit our ‘FIT for screening’ website www.fitscreening.eu/patients

 

 

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CyFlow Cube 8

  • Configurations with up 8 optical parameters (up to 6 colours)
  • Choice of lasers with 375, 407, 488, 532, 561, 594, 638, 640, 785 nm
  • Particle size: 0.1 – 100 µm
  • Fluorescence sensitivity: ≤ 100 MESF (FITC) | ≤ 50 MESF (PE)
  • Optional CyFlow Robby 8 Autoloading Station

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High performance flow cytometry

High-performance flow cytometry

The CyFlow Cube 8 is a compact flow cytometer for the analysis of single cells and microscopic particles with a high grade of integration. The CyFlow Cube 8 offers the unique combination of a truly stand-alone system of a small footprint with a modular configuration concept with up to 6 colours. The easy-to-use CyFlow software provides control of the instrument, data acquisition, analysis and storage. The possibility of customised instrument settings facilitates switching between different applications. CyFlow Cube 8 offers True Volumetric Absolute Counting (TVAC), which allows the display of particle concentrations for any subsets of cells without the need for reference beads, even if defined by a gate at a later time after the acquisition.

* Optional CyFlow Robby 8 Autoloading Station for well plates and tubes

* Optional CyFlow Sorter for closed, non-destructive cell and particle sorting with high purity

Flow Cytometry

Software

Software

CyFlow: Intuitive software for CyFlow Cube 8

The newly designed, intuitive CyFlow acquisition and analysis software offers the measurement of millions of cells at your fingertips.

  • Quickly choose your plots for analysis from all of the combinations shown in Preview Mode.
  • Easily compare plots with the Overlay Wizard, for single or dual parameters.
  • Share and customise your results within your research organisation with the Report Generator.
  • Change your view with Zoom and V-Log Scale; you are even able to gate in Zoom Mode.
  • Compensate rapidly with the Compensation Wizard.
  • CyFlow optimally supports the True Volumetric Absolute Counting of the CyFlow Cube 8, displaying particle concentrations for any subsets of cells, even if defined by a gate at a later time after the acquisition.
Specification

Specification

CyFlow Cube 8 

Light sources:

Up to four light sources (three lasers + high-power UV LED)

  • Blue laser: 50mW at 488nm, adjustable to 200mW at 488nm
  • Red laser: 25mW at 638nm, 40mW at 640nm
  • Violet laser: 100mW at 405nm
  • UV laser: 60mW at 375nm
  • Green laser: 30mW at 532nm, 100mW at 532nm
  • Yellow laser: 100mW at 561nm
  • Orange laser: 50mW at 594nm
  • High-power UV LED: 365nm

 

Optics:

  • Modular optical system with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL6
  • Exchangeable optical filters

 

Flow system:

  • Quartz flow cuvette for laminar sample transport and hydrodynamic focussing
  • True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
  • Computer-controlled precision syringe pump, speed continuously adjustable from 0-20 µL/s

 

Software:

  • Windows™-based FCM software CyFlow for real-time data acquisition, real-time data analysis and real-time data display
  • Easy set-up of experimental templates (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data

 

Dimensions:

  • Stand-alone instrument: W 500mm x H 370mm x D 470mm
  • With Autoloading Station: W 840mm

 

CyFlow Cube 8 Sorter 

General facts:

Configurations with up to five optical parameters (up to three colours)

Sterile sorting by closed fluidic system

Optional CyFlow Robby 8 Autoloading Station for well plates and tubes 

 

Light sources:

Up to two simultaneous light sources (lasers) 

  • Blue laser: 50mW at 488nm, adjustable to 200mW at 488nm
  • Red laser: 25mW at 638nm, 40mW at 640nm
  • Violet laser: 100mW at 405nm
  • UV laser: 60mW at 375nm
  • Green laser: 30mW at 532nm, 100mW at 532nm
  • Yellow laser: 100mW at 561nm
  • Orange laser: 50mW at 594nm

 

Optics:

  • Modular optical system with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL3
  • Exchangeable optical filters

 

Flow system:

  • Quartz flow cuvette for laminar sample transport and sorting process
  • Completely closed and integrated fluidic system
  • True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
  • Computer-controlled precision syringe pump, speed continuously adjustable from 0-20 µL/s

 

Software:

  • Windows™-based FCM software CyFlow for real-time data acquisition, real-time data analysis and real-time data display
  • Easy set-up of experimental templates (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data

 

Dimensions: 

 

  • W 500mm x H 370mm x D 470mm (without sorting module)

 

 

Upgrade

Upgrade

CyFlow Robby 8 Autoloading Station 

 

General facts:

  • Protective housing with no light incidence 
  • Flexible well/tube measurements

 

Microplates and tubes:

  • 48- or 96-well plates; V-, U- and flat-bottom are fitting (max.2) 
  • Plates meeting the standards ANSI/SLAS (1-2004 through 4-2004) 
  • 2mL-tubes (max. 120)

 

Sample volume:

  • Flexible choice of sample volume up to 180 µL for plates and 2,000 µL for tubes

 

Speed:

  • Continuously adjustable from 0.2 µL/s to 10 µL/s
  • Approx. 1h for a 96-well plate (speed 2 µL/s, sample volume 20 µL)

 

Software:

  • CyFlow Windows™-based software

 

Dimensions:

  • W 370mm x H 370mm x D 332mm
Application

Application

Absolute cell and particle counting
Cell and particle sorting

Microbiology: 

  • Yeast viability and fermentation process control 
  • Water quality analysis 
  • Absolute counting of bacteria and other microorganisms 
  • Viability of bacteria and other microorganisms 
  • Quality control in dairy industry and milk products 
  • Marine biology and algae 
  • Agrosciences and aquaculture 
  • Quality control in cosmetics

 

Industrial applications: 

  • Bioreactor process optimisation 
  • Quality assurance in food and beverage industry 
  • Biomonitoring 
  • Biofuels

 

Cell biology: 

  • Cell cycle and DNA analysis 
  • Viability and live/dead analysis 
  • Cell proliferation
  • Apoptosis 
  • Cancer research 
  • Toxicology
  • Cytokines and bead-based assays 
  • Cell signalling 
  • Plant biology 
  • Detection of ploidy level 
  • Plant genome size and DNA analysis 
  • Biomedical research 
  • Animal models 
  • Stem cells 
  • Immunology 
  • Hematological disorders 
  • Platelets
Complementary products

Complementary products

Sample preparation

Contact

Sysmex Europe GmbH

+49 (40) 527 26 0
+49 (40) 527 26 100
info@sysmex-europe.com
Bornbarch 1
22848 Norderstedt
Germany

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