KOMBITEST B/NK Cell 4-color
| Application: | Flow cytometry |
| Regulatory Status: | CE IVD |
| Quantity: | 50 tests |
| Reactivity: | Human |
| Order number: | ED7735 |
What is detected and measured
The KOMBITEST B/NK Cell 4-color reagent is a premixed combination of fluorochrome-labeled monoclonal antibodies intended for detection and enumeration of lymphocyte populations and subsets in human whole blood by flow cytometry.
It detects and measures relative percentages and absolute counts of human T cells (CD3+), B cells (CD3-CD19+) and NK cells (CD3-CD16+56+).
It is intended for use in the immunological assessment of normal patients, and might aid to diagnosis of patients having, or suspected of having, immune deficiency.
Equipment required
A flow cytometer with two laser excitation sources (488 nm and ~635 nm) and appropriate filters and detectors, as described in the IFU.
It is compatible with the Sysmex XF-1600 flow cytometer.
Manufacturer: Exbio Praha, a.s.
The KOMBITEST B/NK Cell 4-colour reagent is a ready-to-use premixed combination of the following fluorochrome-labelled murine monoclonal antibodies, diluted at optimum concentrations in a stabilising phosphate-buffered saline (PBS) solution containing 15 mM sodium azide:
| Antigen | Fluorochrome | Clone | Isotype |
| CD3 | FITC | TB3 | IgG2b |
| CD16 | PE | 3G8 | IgG1 |
| CD56 | PE | LT56 | IgG2a |
| CD19 | APC | LT19 | IgG1 |
| CD45 | PerCP | MEM-28 | IgG1 |
1 vial is sufficient for 50 tests.