Complementary CE IVD-marked product for the MammaTyper kit
Faster procedure eliminates the need for a column system in nucleic acid purification by utilizing magnetic beads.
Environmentally friendly and safer for users as it does not utilize a deparaffinization reagent and reduces plastic material waste.

RNXtract^® Nucleic Acid Extraction Kit

RNXtract^®is intended for routine clinical use for extraction and purification of total nucleic acid from formalin-fixed and paraffin-embedded (FFPE) sample material.

The kit includes eight reagent vials supporting 48 extractions and is validated for use with the MammaTyper® assay.

More details

Graphical Representation of the Procedure

RNXtract^® Nucleic Acid Extraction Kit overview:

Purifies nucleic acids from FFPE samples
Magnetic bead–based binding ensures efficient nucleic acid isolation with high purity and yield.
Optimized washing removes proteins, nucleases, and impurities for high purity
No deparaffinization step is required making the process user-safe and environmentally friendly.
No plastic columns used → reduces nucleic acid loss during purification

Supporting literature:

Varga Z, Lebeau A, Bu H, Hartmann A, Penault-Llorca F, Guerini-Rocco E, Schraml P, Symmans F, Stoehr R, Teng X, Turzynski A, von Wasielewski R, Gürtler C, Laible M, Schlombs K, Joensuu H, Keller T, Sinn P, Sahin U, Bartlett J, Viale G. An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper®. Breast Cancer Res. 2017 May 11;19(1):55. doi: 10.1186/s13058-017-0848-z. PMID: 28490348; PMCID: PMC5426065.

Laible M, Schlombs K, Kaiser K, Veltrup E, Herlein S, Lakis S, Stöhr R, Eidt S, Hartmann A, Wirtz RM, Sahin U. Technical validation of an RT-qPCR in vitro diagnostic test system for the determination of breast cancer molecular subtypes by quantification of ERBB2, ESR1, PGR and MKI67 mRNA levels from formalin-fixed paraffin-embedded breast tumor specimens. BMC Cancer. 2016 Jul 7;16:398. doi: 10.1186/s12885-016-2476-x. PMID: 27389414; PMCID: PMC4936300.

Gürtler C, Laible M, Schwabe W, Steinhäuser H, Li X, Liu S, Schlombs K, Sahin U. Transferring a Quantitative Molecular Diagnostic Test to Multiple Real-Time Quantitative PCR Platforms. J Mol Diagn. 2018 Jul;20(4):398-414. doi: 10.1016/j.jmoldx.2018.02.004. Epub 2018 Apr 3. PMID: 29625248.

Technical specifications

Feature	RNXtract® Nucleic Acid Extraction Kit
Sample type	10 uM FFPE tissue section (tumor cell content >20 %)
Sample capacity	48 extractions
Product Classification	IVDD
Technology	Binding of nucleic acids to magnetic beads
Elution Volume	100 µL
Extraction Time	Approximately 2.5 hours
Final Product	Total Nucleic Acid
Pre-deparaffinization of FFPE	Not required
Storage Conditions	Stable at room temperature for up to 12 months
Catalogue Number	CC01011