Myeloid malignancies are a heterogeneous group of diseases that are associated with a wide range of variants, ranging from single point mutations to structural variations. SureSeq™ Myeloid NGS solutions combine the rapid Universal NGS Workflow Solution hybridisation-based target enrichment with Interpret analysis software in the panel of your choice for accurate detection of key genes implicated in the characterisation of myeloid disorders.

The SureSeq™ Core MPN (+ BCR-ABL) panel

Core content: CALR, JAK2, MPL SNVs and indels down to 1% VAF; customise by adding BCR-ABL translocation
1-day sample-to-sequencer: streamlined library preparation and rapid 30-minute hybridisation

The SureSeq™ Myeloid Plus Workflow

Mid-size content: detect SNVs and indels in 49 genes implicated in myeloid malignancies down to 2.5% VAF (including robust detection of FLT3-ITDs and KMT2A-PTDs), together with 44 SNPs as ID markers and 4 sex chromosome genes

The SureSeq™ Pan-Myeloid panel

Comprehensive content: confidently detect low-frequency SNVs and indels down to 1% VAF in 70 genes implicated in myeloid malignancies, including robust detection of FLT3-ITDs and KMT2A-PTDs

More details

The SureSeq™Core MPN (+ BCR-ABL) panel

Table 1: The SureSeq™ Core MPN Panel targets 4 exons in 3 genes implicated in myeloproliferative neoplasms (MPNs), covering various key MPN driver mutations.

Figure 1. Detection of a 52 bp deletion (type 1) in exon 9 of CALR (bottom panel), compared to a wild-type sample (top panel).

Figure 2. BCR-ABL translocation reported in Interpret.

The SureSeq™ Myeloid Plus Workflow

Table 2: The SureSeq™ Myeloid Plus Workflow targets 49 key genes implicated in myeloid disorders.

Figure 3. FLT3-ITDs of various sizes and regions containing multiple ITDs can be detected confidently.

The SureSeq™Pan-Myeloid panel

Table 3: The SureSeq™ Pan-Myeloid Panel targets 70 genes implicated in a variety of myeloid disorders.

Figure 4. Illustration of the excellent coverage uniformity of the CEBPA gene.

Disclaimer

SureSeq™: For Research Use Only, not for use in diagnostic procedures. Product availability may vary from country to country. Contact your local representatives for availability.

Manufacturer and Trademarks: SureSeq™ (Oxford Gene Technology IP Limited)

Technical specifications

Feature	Core MPN	Myeloid Plus	Pan-Myeloid
Sample capacity	24 or 96 samples per kit	24 or 96 samples per kit	24 or 96 samples per kit
Number of targets	3 genes	49 (+4) gender marker genes	70 myeloid genes and 4 gender marker genes
Panel size	1 kb	132 kb	221 kb
DNA input recommended	>500 ng high-quality DNA	>500 ng high-quality DNA	>500 ng high-quality DNA
Aberration Types	SNV, indel	SNV, indel, ITD, PTD	SNV, indel, ITD, PTD
Limit of detection	SNVs / indels: 1% VAF Translocation (optional)	SNVs/ indels: 2.5 - 5% VAF	SNVs/indels: 1% VAF
Gene list	CALR Exon 9, JAK2 Exons 12 and 14, MPL Exon 10	AMELX, AMELY, ASXL1, BCOR, BCORL1, BRAF, CALR, CBL, CEBPA, CSF3R, CTNNA1, CUX1, DDX41, DNMT3A, ETNK1, ETV6, EZH2, FBXW7, FLT3, GATA2, GNAS, IDH1, IDH2, IKZF1, IRF1, JAK1, JAK2, KIT, KMT2A, KRAS, MPL, MYD88, NOTCH1, NPM1, NRAS, PAX5, PHF6, PPM1D, PTPN11, RAD21, RUNX1, SETBP1, SF3B1, SH2B3, SRSF2, STAG2, TET2, TGIF2LX, TGIF2LY, TP53, U2AF1, WT1 and ZRSR2	ABL1, ASXL1, BCOR, BCORL1, BIRC3, BRAF, BTK, CALR, CBL, CDKN2A, CEBPA, CHEK2, CREBBP, CSF3R, CTNNA1, CUX1, CXCR4, DDX41, DNMT3A, EP300, ETNK1, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GNAS, GNB1, HRAS, IDH1, IDH2, IKZF1, IRF1, JAK1, JAK2, JAK3, KIT, KMT2A, KMT2C, KRAS, MPL, MYD88, NF1, NFE2, NOTCH1, NPM1, NRAS, PAX5, PDGFRA, PHF6, PLCG2, PPM1D, PTEN, PTPN11, RAD21, RUNX1, SETBP1, SF3B1, SH2B3, SRSF2 STAG2, STAT3 and STAT5B

Ordering information

770001-24	SureSeq™ Core MPN Panel
770001-96	SureSeq™ Core MPN Panel
770024-24	SureSeq™ Core MPN + BCR-ABL Panel
770024-96	SureSeq™ Core MPN + BCR-ABL Panel
770002-24	SureSeq™ Myeloid Plus Panel
770002-96	SureSeq™ Myeloid Plus Panel
770003-24	SureSeq™ Pan‐Myeloid Panel
770003-96	SureSeq™ Pan‐Myeloid Panel
770510-24	Universal NGS Workflow Solution V2 (24)
770510-96	Universal NGS Workflow Solution V2 (96)